Computer-Assisted DEgenerate Primer COnstruction



Order 123456789101112131415161718
Seq5'ALPNGHYILRARGAPTAE3'
Name5'AlaLeuProAsnGlyHisTyrIleLeuArgAlaArgGlyAlaProThrAlaGlu3'
Deg (71) 464242236646444442
RNA GCA
GCC
GCG
GCU
CUA
CUC
CUG
CUU
UUA
UUG
CCA
CCC
CCG
CCU
AAC
AAU
GGA
GGC
GGG
GGU
CAC
CAU
UAC
UAU
AUA
AUC
AUU
CUA
CUC
CUG
CUU
UUA
UUG
AGA
AGG
CGA
CGC
CGG
CGU
GCA
GCC
GCG
GCU
AGA
AGG
CGA
CGC
CGG
CGU
GGA
GGC
GGG
GGU
GCA
GCC
GCG
GCU
CCA
CCC
CCG
CCU
ACA
ACC
ACG
ACU
GCA
GCC
GCG
GCU
GAA
GAG
Deg DNA GCI
GCN
YTI
CTN
CTY
TTR
YTR
CCI
CCN
AAYGGI
GGN
CAYTAYATHYTI
CTN
CTY
TTR
YTR
MGI
AGR
CGN
CGY
MGR
GCI
GCN
MGI
AGR
CGN
CGY
MGR
GGI
GGN
GCI
GCN
CCI
CCN
ACI
ACN
GCI
GCN
GAR
Primer-N5'GCIYTICCIAAYGGICAYTAYATHYTIMGIGCIMGIGGIGCICCIACIGCIGAR3'
Compl.3'CGIRAIGGITTRCCIGTRATRTADRAIKCICGIKCICCICGIGGITGICGICTY5'
Primer-C5'YTCIGCIGTIGGIGCICCICKIGCICKIARDATRTARTGICCRTTIGGIARIGC3'
Primer length is 54.
Melting temperature is 64.14°C
Annealing temperature is 59.14°C
Primer degenaracy is = 768
1
YTC
211
2
IGC
111
3
IGT
111
4
IGG
111
5
IGC
111
6
ICC
111
7
ICK
112
8
IGC
111
9
ICK
112
10
IAR
112
11
DAT
311
12
RTA
211
13
RTG
211
14
ICC
111
15
RTT
211
16
IGG
111
17
IAR
112
18
IGC
111
IUPAC table used
CodeC'CodesDeg
ATA---1
BV-CGT3
CG-C--1
DHA-GT3
GC--G-1
HDAC-T3
IIACGT1
KM--GT2
MKAC--2
NNACGT4
RYA-G-2
SS-CG-2
TA---T1
UA---T1
VBACG-3
WWA--T2
YR-C-T2